A procedure for the quantitative determination of protein of the rat heart.
نویسندگان
چکیده
A procedure has been developed for the quantitative determination of the extraetable proteins of the nit heart. Utilizing extraction with a sucrose-versene solution and employing differential centrifugation, followed by extraction with a phosphate-pyrophosphate buffer, low-ionic-strength extractives, a mitochondrial fraction, and high-ionic-strength extractives have been obtained. Electrophoretic analysis has shown five subfractions to be present in the low-ionic-strength extractives and three sub-fractions in the high-ionic-strength extractives.
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ورودعنوان ژورنال:
- Circulation research
دوره 3 5 شماره
صفحات -
تاریخ انتشار 1955